The combined double substitutions, I86A L90A and I86A A91W, showed an additive effect on BAK-mediated MOMP and active BAK conformation, further disabling BID activation of BAK (Fig. 3b, d ...
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Get QuoteThe sequence of the fluorescently-labeled Bak peptide is FITC-Ahx-GQVGRQLAIIGDDINR-CONH) 5 × 10 REN cells were exposed to 5 μM JY-1-106, 20 μM ABT-737 or a DMSO control for 12 hours and the cells were then harvested and lysed. Bcl- x and Bak were immunoprecipitated with specific antibodies.
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